Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44

last updated: 2021-01-15
ProjectCHEM2NATURE :: publications list
TitleTunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
Publication TypePapers in Scientific Journals
Year of Publication2020
AuthorsAmorim S., Pashkuleva I., Reis C. A., Reis R. L., and Pires R. A.

We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) – a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.

JournalJournal Of Materials Chemistry B
Date Published2020-03-20
PublisherThe Royal Society of Chemistry
Keywordsbioactivity, CD44, Hyaluronic acid
Peer reviewedyes

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