Project | CARTI-LIKE :: publications list |
Title | Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2019 |
Authors | Martín-Pastor M., Ferreira A., Mopert X., Nunes C., Coimbra M. A., Reis R. L., Guezennec J., and Novoa-Carballal R. |
Editors | Elsevier |
Abstract | MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications. |
Journal | Carbohydrate Polymers |
Volume | 222 |
Pagination | 114999 |
Date Published | 2019-07-04 |
Publisher | Elsevier |
ISSN | 0144-8617 |
DOI | 10.1016/j.carbpol.2019.114999 |
URL | https://doi.org/10.1016/j.carbpol.2019.114999 |
Keywords | Polysaccharide properties, vibrio |
Rights | restrictedAccess |
Peer reviewed | yes |
Status | published |