Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio

last updated: 2022-06-27
ProjectCARTI-LIKE :: publications list
TitleStructure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio
Publication TypePapers in Scientific Journals
Year of Publication2019
AuthorsMartín-Pastor M., Ferreira A., Mopert X., Nunes C., Coimbra M. A., Reis R. L., Guezennec J., and Novoa-Carballal R.
EditorsElsevier
Abstract

MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.

JournalCarbohydrate Polymers
Volume222
Pagination114999
Date Published2019-07-04
PublisherElsevier
ISSN0144-8617
DOI10.1016/j.carbpol.2019.114999
URLhttps://doi.org/10.1016/j.carbpol.2019.114999
KeywordsPolysaccharide properties, vibrio
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

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