Multifunctional calcium-based nanocarriers for synergistic treatment of triple-negative breast cancer

last updated: 2024-06-28
ProjectOncoNeoTreat :: publications list
TitleMultifunctional calcium-based nanocarriers for synergistic treatment of triple-negative breast cancer
Publication TypePapers in Scientific Journals
Year of Publication2024
AuthorsMartins S. A., Costa R. R., Brito A., Reis R. L., Alves N. M., Pashkuleva I., and Soares da Costa D.

Targeted breast cancer therapies hold the potential to improve the efficiency of drug delivery to the pathology site without impacting the viability and function of healthy cells. Herein, we developed multifunctional nanocarriers that target simultaneously several downstream signaling processes in triple negative breast cancer cells. The system comprises pH sensitive CaCO3 nanoparticles (NPs) as carriers of the anticancer drug doxorubicin (DOX). The NPs were coated in a layer-by-layer (LbL) fashion using poly-l-lysine and hyaluronic acid to target receptors overexpressed in breast cancer (e.g. CD44, RHAMM). Spheroids of the triple-negative Hs578T cell line were used as a 3D model to assess the therapeutic potential of this system. Our results showed that the NPs act via a synergistic mechanism that combines Ca2+ overload causing cell calcification and DNA damage by DOX. The LbL coating was crucial for the protection of the healthy cells, i.e. it provides NPs with targeting capacity. The overall data suggests that the LbL-coated NPs loaded with DOX hold great potential for the treatment of breast cancer.

JournalJournal Of Colloid And Interface Science
Pagination500 - 512
Date Published2024-06-23
Keywordsbreast cancer, Calcium overload, cell spheroids, Core/shell nanoparticles, doxorubicin, Hyaluronic acid
Peer reviewedyes

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