Mineralization of layer-by-layer ultrathin films containing microfluidic-produced hydroxyapatite nanorods

last updated: 2019-11-12
ProjectELASTISLET :: publications list
TitleMineralization of layer-by-layer ultrathin films containing microfluidic-produced hydroxyapatite nanorods
Publication TypePapers in Scientific Journals
Year of Publication2019
AuthorsRial R., Costa R. R., Reis R. L., Liu Z., Pashkuleva I., and Ruso J. M.

We describe the assembly of layer-by-layer (LbL) ultrathin films containing bioactive hydroxyapatite (HAp) rod-shaped nanoparticles with mineralizing capacity. Monodisperse 96 nm long and 9 nm wide HAp nanorods with a surface charge of –14 mV were produced with a microfluidic system. The negatively charged HAp nanorods were assembled with the polycation poly-L-lysine (PLL) in LbL fashion. The successful deposition of alternating layers was confirmed by quartz-crystal microbalance with dissipation monitoring. The Voigt-based viscoelastic model demonstrated steady film growth where three PLL/HAp bilayers reached a thickness of 70 nm. The bioactivity of [PLL/HAp]3 was evaluated in vitro by following the formation of a mineralized hydroxyapatite layer in simulated body fluid (SBF). X-ray diffraction, energy-dispersive X-ray spectroscopy and scanning electron microscopy (SEM) demonstrated formation of a crystalline hydroxyapatite layer and complete surface coverage within 7 days. SaOs-2 osteoblasts-like cells attached to the mineralized surfaces and developed longer filopodia extensions when compared to non-mineralized samples. Our results showed that [PLL/HAp]3 films are feasible osteoconductive coatings applicable to orthopedic implants and fixation devices.

JournalCrystal Growth & Design
Pagination6351 - 6359
Date Published2019-09-25
Keywordselectrostatic interactions, hydroxyapatite, layer-by-layer, Microfluidics, osteoconductivity
RightsembargoedAccess (1 Year)
Peer reviewedyes

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