Isolation methods of human and bovine adipose tissue derived stem cells

The human term placenta is a fetomaternal organ that has attracted much interest during the last years due to its interest as stem cell source. Moreover, cells from placenta are associated with early embryonic origin and with immunomodulatory properties. Thus, theoretically, MSCs from maternal and fetal origin can be isolated from this tissue. In this work, the amniotic membrane was explored as a source of mesenchymal stem cells (MSCs). Isolated cells were characterized based on the guidelines stated in the consensus from the “First International Workshop on Placental-derived Stem cells” [1], namely for their adherence to plastic, morphological and phenotypical features.

Results showed that isolated cells present the ability to develop fibroblast colony-forming units (CFU-Fs), displayed fibroblast-like morphology and adherence to tissue culture plastic. Surface antigen expression of typical MSCs markers such as CD90, CD73 and CD105 were found to be positive, with the absence of CD45, CD34, CD14, and HLADR. Moreover, isolated cells exhibited a population of CD49e⁺ and CD49f⁺, characteristic of amniotic-derived stem cells [2]. Additionally, cells differentiation potential onto osteogenic, adipogenic and chondrogenic lineages was also demonstrated.

These cells will now be exploited for regenerative medicine purposes, taking advantage of their immunomodulatory potential.

1.         Parolini, O., et al., Concise review: isolation and characterization of cells from human term placenta: outcome of the first international Workshop on Placenta Derived Stem Cells. Stem Cells, 2008. 26(2): p. 300-11.

2.         Pozzobon, M., M. Piccoli, and P. De Coppi, Stem cells from fetal membranes and amniotic fluid: markers for cell isolation and therapy. Cell Tissue Bank, 2014. 15(2): p. 199-211.