Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development

last updated: 2020-02-26
ProjectFoReCaST :: publications list
TitleBioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development
Publication TypePapers in Scientific Journals
Year of Publication2020
AuthorsSoares da Costa D., Sousa J. C., da Mesquita S., Petkova-Yankova N., Marques F., Reis R. L., Sousa N., and Pashkuleva I.
EditorsFerro V.
Abstract

The expression of different glycans at the cell surface dictates cell interactions with their environment and other cells, being crucial for the cell fate. The development of the central nervous system is associated with tremendous changes in the cell glycome that is tightly regulated. Herein, we have employed biorthogonal Cu-free click chemistry to image temporal distribution of different glycans in live mouse hippocampal neurons during their maturation in vitro. We show development-dependent glycan patterns with increased fucose and decreased mannose expression at the end of the maturation process. We also demonstrate that this approach is biocompatible and does not affect glycan transport although it relies on an administration of modified glycans. The applicability of this strategy to tissue sections unlocks new opportunities to study the glycan dynamics under more complex physiological conditions.

JournalMolecules
Volume25
Issue4
Pagination795
Date Published2020-02-12
PublisherMDPI
ISSN1420-3049
DOI10.3390/molecules25040795
URLhttps://www.mdpi.com/1420-3049/25/4/795
Keywordsbioorthogonal chemistry, GLYCOSYLATION, Imaging, neuronal development
RightsopenAccess
Peer reviewedyes
Statuspublished

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