Volatile composition of Catharanthus roseus (L.) G. Don using solid-phase microextraction and gas chromatography/mass spectrometry

last updated: 2014-05-20
TitleVolatile composition of Catharanthus roseus (L.) G. Don using solid-phase microextraction and gas chromatography/mass spectrometry
Publication TypePapers in Scientific Journals
Year of Publication2009
AuthorsGuedes de Pinho P., Gonçalves R. F., Valentão P., Pereira D. M., Seabra R. M., Andrade P. B., and Sottomayor M.
Abstract

A total of 88 volatile and semi-volatile components were formally or tentatively identified in flowers, leaves and stems of Catharanthus roseus (L.) G. Don (cv. Little Bright Eye), by headspace solid-phase microextraction (HS-SPME) and by dichloromethane extraction, combined with gas chromatography–mass spectrometry (GC–MS). These include some diterpenic compounds (manool and manoyl oxides), a sesquiterpen (α-bisabolol), and some pyridine, pyrazine, indol and carotenoid derivatives. Applying multivariate analysis (principal component analysis and agglomerative hierarchic cluster analysis) to the HS-SPME–GC–MS data, it was possible to characterize each part of the vegetal material using a relative small number of compounds. Hence, flowers were richer in terpenic molecules (including limonene), α-bisabolol, methyljasmonate, cis-jasmone, 2-phenylethanol, phenylacetaldehyde, trans-2-octenal, benzylic alcohol and 2-isobutyl-3-methoxypyrazine. Leaves can be characterized by the methyl and propyl esters of fatty acids, mono- and disaturated, trans-phytol, carotenoid derivative compounds, hydrofarnesylacetone, methylanthranilate, manool and epi-manool oxide, while stems have high levels of volatile aldehydes, such as hexanal, octanal, cis-2-nonenal, cis-2-decenal, cistrans-2,6-nonadienal, transtrans-2,4-decadienal andcistrans-2,4-decadienal. Dichloromethane extraction allowed also the identification of some alkaloid-like compounds that were not detected by HS-SPME.

JournalJournal of Pharmaceutical & Biomedical Analysis
Volume49
Pagination674-685
Date Published2009-04-05
KeywordsGC–MS, HS-SPME, Volatiles and semi-volatiles
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

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