Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere

last updated: 2013-11-13
TitlePorous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
Publication TypePapers in Scientific Journals
Year of Publication2013
AuthorsSilva S. P., Moreira-Silva J., Silva T. H., Perez-Martin R. I., Sotelo C. G., Mano J. F., Duarte A. R. C., and Reis R. L.
Abstract

The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare pre-scaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.

JournalMacromolecular Bioscience
Volume13
Issue11
Pagination1621-1631
Date Published2013-08-30
PublisherWiley
URLhttp://onlinelibrary.wiley.com/doi/10.1002/mabi.201300228/abstract
KeywordsCollagen, Hydrogels, marine biotechnolgoy, supercritical fluids, Tissue engineering
RightsopenAccess
Peer reviewedyes
Statuspublished

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