Title | Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2013 |
Authors | Silva S. P., Moreira-Silva J., Silva T. H., Perez-Martin R. I., Sotelo C. G., Mano J. F., Duarte A. R. C., and Reis R. L. |
Abstract | The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare pre-scaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering. |
Journal | Macromolecular Bioscience |
Volume | 13 |
Issue | 11 |
Pagination | 1621-1631 |
Date Published | 2013-08-30 |
Publisher | Wiley |
URL | http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300228/abstract |
Keywords | Collagen, Hydrogels, marine biotechnolgoy, supercritical fluids, Tissue engineering |
Rights | openAccess |
Peer reviewed | yes |
Status | published |