Pericytes contribute to the stability of the vascular network of osteogenic tissue formed from cell sheet-based constructs

last updated: 2013-04-10
TitlePericytes contribute to the stability of the vascular network of osteogenic tissue formed from cell sheet-based constructs
Publication TypeConference Abstract -ISI Web of Science Indexed
Year of Publication2012
AuthorsMendes L. F., Pirraco R. P., Szymczyk W., Santos T. C., Frias A. M., Reis R. L., and Marques A. P.

Despite the increased use of cell sheets for tissue engineering, the appli- cation of this new technology for thick tissue reconstruction is being limited by old barriers such as the need to create a pre-vascular net- work to ensure proper nutrient and oxygen supply in vivo. In the pres- ent work, we created a cell sheet-based construct by co-culturing bone marrow-derived osteogenic and pericyte-like cells with human umbili- cal vein endothelial cells (HUVECs) with the purpose of enhancing the in vivo vascularization of newly formed osteogenic tissue. Human bone marrow mesenchymal stromal cells (hBMSCs) were isolated and cul- tured in medium supplemented with osteogenic factors or TGF-b1 to obtain either osteogenic or CD146+ pericyte-like cells. Immunocyto- chemistry analysis of the co-cultures showed organized structures formed by CD146+ hBMSCs and HUVECs over the osteogenic cell sheet, suggesting the existence of cross-talk between the co-cultured cell types. Nude mice were used to test the ability of those constructs to form functional and vascularized osteogenic tissue. Immunohisto- chemistry analysis of cell sheet-based constructs after 1 and 3 weeks of transplantation revealed the integration of HUVECs with neighboring host’s vessels. Additionally, the analysis of the diameter of the blood vessels showed a higher mean diameter for the condition that com- bined pericyte-like cells and HUVECs, reinforcing the advantage of the proposed model regarding blood vessels maturation and stability.

JournalJournal of Tissue Engineering and Regenerative Medicine
Date Published2012-09-09
PublisherJohn Wiley & Sons
Conference Location3rd World Congress of the Tissue Engineering and Regenerative Medicine International Society, Vienna, Austria
Keywordscell sheets, endothelial cells, osteoblasts, pericytes
Peer reviewedyes

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