Osteogenic Differentiation of Adipose Stem Cells by Endothelial Cells Co-culture Within Liquified Capsules

last updated: 2015-09-23
ProjectLA ICVS/3Bs - 2015-2017 :: publications list
TitleOsteogenic Differentiation of Adipose Stem Cells by Endothelial Cells Co-culture Within Liquified Capsules
Publication TypeConference Abstract -ISI Web of Science Indexed
Year of Publication2015
AuthorsCorreia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., and Mano J. F.
Abstract

Osteogenic Differentiation of Adipose Stem Cells by Endothelial Cells Co-culture Within Liquified Capsules

Clara R. Correia1,2, Rogério P. Pirraco1,2, Mariana T. Cerqueira1,2, Alexandra P. Marques1,2, Rui L. Reis1,2, João F. Mano1,2 

13B’s Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal

2ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, Portugal

 

Inspired by the native co-existence of multiple cell types and from the concept of deconstructing the “stem cell niche”, we propose a co-encapsulation strategy within liquified capsules. The present team has already proven the application of liquified capsules as bioencapsulation systems1. Here, we intend to use the optimized system towards osteogenic differentiation. Capsules encapsulating adipose stem cells alone (MONO-capsules) or in co-culture with endothelial cells (CO-capsules) were maintained in endothelial medium with or without osteogenic differentiation factors. The suitability of the capsules for living stem and endothelial cells encapsulation was demonstrated by MTS and DNA assays. The osteogenic differentiation was assessed by quantifying the deposition of calcium and the activity of ALP up to 21 days. CO capsules had an enhanced osteogenic differentiation, even when cultured in the absence of osteogenic factors. Furthermore, osteopontin and CD31 could be detected, which respectively indicate that osteogenic differentiation had occurred and endothelial cells maintained their phenotype. An enhanced osteogenic differentiation by co-encapsulation was also confirmed by the upregulation of osteogenic markers (BMP-2, RUNX2, BSP) while the expression of angiogenic markers (VEGF, vWF, CD31) revealed the presence of endothelial cells. The proposed capsules can also act as a growth factor release system upon implantation, as showed by VEGF and BMP-2 quantification. These findings demonstrate that the co-encapsulation of stem and endothelial cells within liquified injectable capsules provides a promising strategy for bone tissue engineering.

 

Acknowledgements

Portuguese-FCT (PhD-grant-SFRH/BD/69529/2010-ClaraCorreia, Post-doctoral-grant-SFRH/BPD/96611/2013-MarianaCerqueira), RL3TECTNORTE010124FEDER000020-RogérioPirraco, and the ERC (grant agreement-ERC-2012-ADG-20120216321266-ComplexiTE).

 

References

1. Correia CR et al. Multilayered Hierarchical Capsules Providing Cell Adhesion Sites. Biomacromolecules 2013;14:743.

JournalTissue Engineering Part A
Conference Name2015 4th TERMIS World Congress
Volume21
IssueS1
PaginationS1-S413
Date Published2015-08-28
PublisherMary Ann Libert, Inc.
Conference LocationBoston, Massachusetts, USA
DOI10.1089/ten.tea.2015.5000.abstracts
URLhttp://online.liebertpub.com/doi/full/10.1089/ten.tea.2015.5000.abstracts
KeywordsCAPSULES, Co-culture, endothelial cells, microparticles, osteogenic differentiation, Stem cells
RightsopenAccess
Peer reviewedyes
Statuspublished

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