Extracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment

last updated: 2016-12-14
ProjectRL3 – TECT :: publications list
TitleExtracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment
Publication TypePapers in Scientific Journals
Year of Publication2016
AuthorsMartins M., Ribeiro D., Martins A., Reis R. L., and Neves N. M.
Abstract

The effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (∼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2SP7SPP1BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors.

JournalStem Cell Reports
Volume6
Issue3
Pagination284-291
Date Published2016-03-08
PublisherCell Press
ISSN2213-6711
DOI10.1016/j.stemcr.2016.01.001
URLhttp://www.cell.com/stem-cell-reports/pdf/S2213-6711(16)00002-3.pdf
Keywordsadvanced therapies/ bone regeneration, DIFFERENTIATION, exosome, osteogenic commitment strategies, Secretome, Stem cells
RightsopenAccess
Peer reviewedyes
Statuspublished

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