Title | Anti-inflammatory effect of unsaturated fatty acids and ergosta-7,22-dien-3-ol from Marthasterias glacialis: prevention of CHOP-mediated ER-stress and NF-κB activation |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2013 |
Authors | Pereira D. M., Correia-da-Silva G., Valentão P., Teixeira N., and Andrade P. B. |
Abstract | There has been increasing awareness to the potential interest of drug discovery from marine natural products to treat several pathological conditions, including inflammation. In this work we describe the anti-inflammatory activity of several compounds present in the echinoderm Marthasterias glacialis (spiny sea-star), using the inflammatory model RAW 264.7 cells challenged with LPS. Lipidomic profiling of the organism revealed two major classes of compounds: fatty acids and sterols. Among these, the predominant compounds cis 11-eicosenoic and cis 11,14 eicosadienoic acids and the unsaturated sterol ergosta-7,22-dien-3-ol were evaluated. The mechanism of action of the compounds was distinct as they modulated different levels of the inflammation pathway. Classical inflammatory markers such as COX-2, iNOS, IL-6 and NF-κB were evaluated. We also studied the contribution of the CHOP pathway-mediated ER-stress to the inflammatory process. Overall, the sterol ergosta-7,22-dien-3-ol was the most active compound, however maximum activity was obtained when all compounds were tested in combination, thus suggesting a potentially synergistic activity of both classes of metabolites. This work establishes the echinoderm M. glacialis as an interesting source of anti-inflammatory molecules. |
Journal | Plos One |
Date Published | 2013-12-20 |
DOI | DOI 10.1371/journal.pone.0088341 |
Keywords | 22-dien-3-ol, Anti-inflammatory, CHOP, Ergosta-7, IKB-α |
Rights | openAccess |
Peer reviewed | yes |
Status | published |