Title | Ultrasound-assisted biosynthesis of novel methotrexate-conjugates |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2018 |
Authors | Noro J., Reis R. L., Cavaco-Paulo A., and Silva C. |
Abstract | New methotrexate-acylglycerols and methotrexate-cyclodextrins (α, β and γ-CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy. |
Journal | Ultrasonics Sonochemistry |
Volume | 48 |
Pagination | 51-56 |
Date Published | 2018-05-17 |
Publisher | Elsevier |
ISSN | 1350-4177 |
DOI | 10.1016/j.ultsonch.2018.05.017 |
URL | https://www.sciencedirect.com/science/article/pii/S1350417718302682?via%3Dihub |
Keywords | Biosynthesis, cyclodextrins, Lipases, Methotrexate, Triacylglycerols, Ultrasound |
Rights | openAccess |
Peer reviewed | yes |
Status | published |