Ultrasound-assisted biosynthesis of novel methotrexate-conjugates

last updated: 2019-01-24
TitleUltrasound-assisted biosynthesis of novel methotrexate-conjugates
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsNoro J., Reis R. L., Cavaco-Paulo A., and Silva C.
Abstract

New methotrexate-acylglycerols and methotrexate-cyclodextrins (α, β and γ-CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy.

JournalUltrasonics Sonochemistry
Volume48
Pagination51-56
Date Published2018-05-17
PublisherElsevier
ISSN1350-4177
DOI10.1016/j.ultsonch.2018.05.017
URLhttps://www.sciencedirect.com/science/article/pii/S1350417718302682?via%3Dihub
KeywordsBiosynthesis, cyclodextrins, Lipases, Methotrexate, Triacylglycerols, Ultrasound
RightsopenAccess
Peer reviewedyes
Statuspublished

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