Silk fibroin/cholinium gallate-based architectures as therapeutic tools

last updated: 2022-06-20
ProjectNature4Health :: publications list
TitleSilk fibroin/cholinium gallate-based architectures as therapeutic tools
Publication TypePapers in Scientific Journals
Year of Publication2022
AuthorsGomes J. M., Silva S. S., Fernandes E. M., Lobo F. C. M., Martín-Pastor M., Taboada P., and Reis R. L.

The combination of natural resources with biologically active biocompatible ionic liquids (Bio-IL) is presented as a combinatorial approach for developing tools to manage inflammatory diseases. Innovative biomedical solutions were constructed combining silk fibroin (SF) and Ch[Gallate], a Bio-IL with antioxidant and anti-inflammatory features, as freeze-dried 3D-based sponges. An evaluation of the effect of the Ch[Gallate] concentration (≤3% w/v) on the SF/Ch[Gallate] sponges was studied. Structural changes observed on the sponges revealed that the Ch[Gallate] presence positively affected the β-sheet formation while not influencing the silk native structure, which was suggested by the FTIR and solid-state NMR results, respectively. Also, it was possible to modulate their mechanical properties, antioxidant activity and stability/degradation in an aqueous environment, by changing the Ch[Gallate] concentration. The architectures showed high water uptake ability and a weight loss that follows the controlled Ch[Gallate] release rate studied for 7 days. Furthermore, the sponges supported human adipose stem cells growth and proliferation, up to 7 days. TNF-α, IL-6 (pro-inflammatory) and IL-10 (anti-inflammatory) release quantification from a human monocyte cell line revealed a decrease in the pro-inflammatory cytokines concentrations in samples containing Ch[Gallate]. These outcomes encourage the use of the developed architectures as tissue engineering solutions, potentially targeting inflammation processes.

JournalActa Biomaterialia
Date Published2022-05-16
PublisherElsevier Ltd
KeywordsAnti-inflammatory properties. Cholinium gallate, Antioxidant properties, Biocompatible ionic liquid, Silk Fibroin
Peer reviewedyes

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