A silk fibroin hydrogel with reversible sol–gel transition

In this report, a novel silk fibroin hydrogel with a reversible sol–gel transition capacity is presented, in which
the base material of this reversible hydrogel is hydrophilic silk fibroin (HSF) obtained by immersing a dried
regenerated Bombyx mori silk fibroin (SF) condensate in deionized water (DI water) and collecting its
lixivium. The resulting HSF sol can exhibit an enhanced sol–gel transition within several hours at suitable
temperatures (25–50 C) without any exterior additive, even at extremely low concentrations (<0.2%, w/
v). The HSF gel can perform thixotropic, reversible gel–sol transitions triggered by a facile cycled shearthinning and resting procedure. Reversible sol–gel transition kinetics analyses and dynamic measurements of the micro-structure transformation during the transition demonstrate that both the bsheet
self-assembling process and metastable hydrogen bonding (H-bond) interactions among these large scale b-sheet aggregates play essential roles in the significant enhancement of the reversible HSF
sol–gel transition. Due to the reversible, thixotropic sol–gel transitions and suitable viscoelasticity of its
shear-thinning system for matching random sizes/shapes, the HSF system is possibly an alternative
injectable hydrogel for applications in 3-dimensional (3D) cell culture and tissue repair in situ.