Project | OsteoCart :: publications list |
Title | Osteogenesis evaluation of duck’s feet derived collagen/hydroxyapatite sponges immersed in dexamethasone |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2017 |
Authors | Kook Y. J., Lee D. H., Song J. E., Tripathy N., Jeon Y. S., Oliveira J. M., Reis R. L., and Khang G. |
Abstract | Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone. Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(−)DC and Dex(−)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(−)DC, Dex(−)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR). Results: In this study, we assessed osteogenic differentiation of BMSCs on Duck’s feet-derived collagen (DC)/ HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days. Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications. |
Journal | Biomaterials Research |
Volume | 21 |
Issue | 2 |
Pagination | 1-7 |
Date Published | 2017-01-02 |
Publisher | BioMed Central |
ISSN | 2055-7124 |
DOI | 10.1186/s40824-017-0088-4 |
Keywords | bone, Collagen, hydroxyapatite |
Rights | openAccess |
Peer reviewed | yes |
Status | published |