Monocyte-derived macrophage immune interactions with gellan gum-based hydrogel formulations

last updated: 2021-11-26
ProjectECM_INK :: publications list
TitleMonocyte-derived macrophage immune interactions with gellan gum-based hydrogel formulations
Publication TypeComunications - Poster
Year of Publication2021
AuthorsMacedo J. A., da Silva L. P., Reis R. L., and Marques A. P.

Gellan gum (GG) hydrogels have been widely used for tissue engineering and regenerative medicine approaches with minimal biocompatibility issues [1]. Biofunctionalization of GG with cell adhesive sites has shown improved in vitro cell response [2], but how it affects the immune response remains to be elucidated. Therefore, in this work, we intended to evaluate the impact of the absence/presence of cell adhesive sites on human monocyte-derived macrophages (MDM) response. MDM, differentiated from primary monocytes sorted from peripheral blood mononuclear cells, were encapsulated in GG hydrogels with or without the cell adhesive site RGD [2]. Cells showed a round-shape morphology in non-adhesive hydrogels and a spread adhesive-like morphology in RGD-containing hydrogels, while cell viability up to 12 days of encapsulation was not affected. A strong release of both proinflammatory (IL-1β, IL-6, IL-12p40, TNF-a) and antiinflammatory (IL-1RA, IL-10, CCL22) associated cytokines was observed 1 day after MDM encapsulation, independently of the hydrogel formulation. This release on day 3 was strongly reduced for most cytokines to undetectable levels for TNF-a. Conversely, CCL17, which was undetectable on day 1, was detected on day 3 even if at very low levels, and CCL22 release slightly increased.  A dependence on the hydrogel formulation was observed, as higher amounts of proinflammatory cytokines (IL-1β and IL-12p40) were detected from MDM within soft hydrogels lacking RGD, whereas a higher release of antiinflammatory cytokines (IL-1RA, IL-10, CCL17, and CCL22) was detected from MDM within RGD-containing hydrogels. Overall, this work reveals that MDM present an immediate but transient response to GG hydrogels, which is affected by its mechanical properties and the presence of a cell-adhesive site.

Acknowledgments: ERC consolidator grant ECM_INK (ERC-2016-COG-d726061), FCT grant (2020.01541.CEECIND/CP1600/CT0024), Project grant PTDC/BTM-ORG/31564/2017.


[1] L. R. Stevens et al. (2016). Biomater. Sci., 2016, 4, 1276.

[2] L. P. da Silva, et al. (2018). Advance Healthcare Materials, 7(5).

Conference NameTERMSTEM
Date Published2021-10-27
Conference LocationPorto, Portugal
KeywordsGellan Gum, hydrogel, immune reactions, macrophage
Peer reviewedno

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