Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging

last updated: 2023-03-28
ProjectNanoTech4ALS :: publications list
TitleMn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging
Publication TypeInvited Journal Paper
Year of Publication2023
AuthorsVieira S., Strymecka P., Stanaszek L., Silva-Correia J., Drela K., Fiedorowicz M., Malysz-Cymborska I., Janowski M., Reis R. L., Lukomska B., Walczak P., and Oliveira J. M.

This work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBPshi/shi/rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures.

Date Published2023-03-28
KeywordsCell delivery, Gellan Gum, injectable hydrogels, magnetic resonance imaging, manganese
Peer reviewedyes

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