Human tendon-derived cell sheets created by magnetic force-based tissue engineering hold tenogenic and immunomodulatory potential

last updated: 2021-07-27
ProjectMagTendon :: publications list
TitleHuman tendon-derived cell sheets created by magnetic force-based tissue engineering hold tenogenic and immunomodulatory potential
Publication TypePapers in Scientific Journals
Year of Publication2021
AuthorsVinhas A., Gonçalves A. I., Rodrigues M. T., and Gomes M. E.
Abstract

Cell sheet technology and magnetic based tissue engineering hold the potential to become instrumental in developing magnetically responsive living tissues analogues that can be potentially used both for modeling and therapeutical purposes. Cell sheet constructions more closely recreate physiological niches, through the preservation of contiguous cells and cell-ECM interactions, which assist the cellular guidance in regenerative processes.

We herein propose to use magnetically assisted cell sheets (magCSs) constructed with human tendon-derived cells (hTDCs) and magnetic nanoparticles to study inflammation activity upon magCSs exposure to IL-1β, anticipating its added value for tendon disease modeling.

Our results show that IL-1β induces an inflammatory profile in magCSs, supporting its in vitro use to enlighten inflammation mediated events in tendon cells. Moreover, the response of magCSs to IL-1β is modulated by pulsed electromagnetic field (PEMF) stimulation, favoring the expression of anti-inflammatory genes, which seems to be associated to MAPK(ERK1/2) pathway. The anti-inflammatory response to PEMF together with the immunomodulatory potential of magCSs opens new perspectives for their applicability on tendon regeneration that goes beyond advanced cell based modeling.

JournalActa Biomaterialia
Pagination1-40
Date Published2021-06-27
ISSNS1742-7061(21)00410-4
DOI10.1016/j.actbio.2021.06.036
URL https://doi.org/10.1016/j.actbio.2021.06.036
KeywordsInflammation, Magnetic cell sheets, Magnetic nanoparticles, Tendon derived cells
RightsopenAccess
Peer reviewedno
Statuspublished

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