Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells

last updated: 2020-09-28
ProjectTERM - Programa Doutoramento Norte 2020 :: publications list
TitleFucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells
Publication TypePapers in Scientific Journals
Year of Publication2020
AuthorsOliveira C., Soares A. I., Neves N. M., Reis R. L., Marques A. P., Tiago S. H., and Martins A.
Abstract

The use of fucoidan, a marine-origin bioactive polymer, is herein proposed as a component of an innovative and effective strategy against melanoma, one of the most aggressive skin cancers. First, fucoidan antitumor activity, in its soluble form, was assessed presenting increased cytotoxicity over melanoma cells when compared to human dermal fibroblasts and keratinocytes. After this antitumor activity validation and trying to develop a more targeted and local strategy aiming to diminish the cytotoxic effects over noncancer cells, fucoidan was immobilized at the surface of an electrospun nanofiber mesh (NFM_Fu), envisioning the development of a therapeutic patch. The maximum immobilization concentration was 1.2 mg mL–1, determined by the Toluidine Blue Assay and confirmed by XPS. Furthermore, NFM_Fu is more hydrophilic than NFM, presenting a contact angle of 36°, lower than the 121° of the control condition. NFM_Fu was able to reduce human melanoma cell viability by 50% without affecting human dermal fibroblasts and keratinocytes. Taken together, these results set the basis for a valuable approach for melanoma treatment.

JournalBiomacromolecules
Volume21
Issue7
Pagination2745–2754
Date Published2020-05-18
PublisherACS Publications
ISSN1525-7797
DOI10.1021/acs.biomac.0c00482
URLhttps://pubs.acs.org/doi/10.1021/acs.biomac.0c00482
KeywordsFucoidan, Marine natural products, melanoma, nanofiber mesh, surface functionalization
RightsopenAccess
Peer reviewedyes
Statuspublished

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