Fish sarcoplasmic proteins as a high value marine material for wound dressing applications

last updated: 2018-04-27
ProjectSPARTAN :: publications list
TitleFish sarcoplasmic proteins as a high value marine material for wound dressing applications
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsVieira S. F., Franco A. R., Fernandes E. M., Amorim S., Ferreira H., Pires R. A., Reis R. L., Martins A., and Neves N. M.

Fish sarcoplasmic proteins (FSP) constitute around 25–30% of the total fish muscle protein. As the FSP are water soluble, FSP were isolated from fresh cod (Gadus morhua) by centrifugation. By SDS-PAGE was possibly determined the composition of FSP extracts (FSP-E). The FSP-E denature at 44.12 ± 2.34˚C, as characterized by differential scanning calorimetry thermograms (DSC). The secondary structure of FSP-E is mainly composed by α-helix structure, as determined by circular dichroism. The cytocompatibility of FSP-E, at concentrations ranging from 5 to 20 mg/mL, was also investigated. For concentrations lower than 10 mg/mL, no cytotoxicity was observed over 72h of fibroblasts culture. Further on, FSP membranes (FSP-M) were produced by spin coating technique to evaluate its properties. FSP-M had uniform surface as analyzed by SEM, and the amount of α-helix structure increased when compared with the FSP-E. The FSP-M are more stable than the FSP-E, since they presented a denaturation temperature of 58.88 ± 3.36˚C, according to the DSC thermogram. FSP-M shown distinctive mechanical properties, with a stiffness of 16.57 ± 3.95 MPa and a yield strength of 23.85 ± 5.97 MPa. Human lung fibroblasts (MRC-5 cell line) cultured in direct contact with FSP-M demonstrate to be cytocompatible for 48h. Based on these results, FSP can be considered a potential biomaterial, recovered from the nature, for wound dressing applications.

JournalColloids and Surfaces B: Biointerfaces
Date Published2018-07-01
KeywordsCytocompatibility, membranes, Physico-chemical characterization, Sarcoplasmic proteins, Spin coating
RightsembargoedAccess (1 Year)
Peer reviewedyes

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