Evaluation of tenocyte response to magnetically actuated biomaterials in experimentally induced model of tendon inflammation

last updated: 2018-02-08
ProjectTERM - Programa de Doutoramento FCT :: publications list
TitleEvaluation of tenocyte response to magnetically actuated biomaterials in experimentally induced model of tendon inflammation
Publication TypeComunications - Poster
Year of Publication2017
AuthorsVinhas A., Rodrigues M. T., Gonçalves A. I., Berdecka D., Reis R. L., and Gomes M. E.

Tendon disorders are frequent and are responsible for substantial morbidity both in sports and in the workplace [1]. Although the mechanisms that weaken the tendon are poorly understood, most tendon disorders are caused by an abnormal/failure regenerative process. However, the involvement of inflammatory cues in the development of tendon injuries is expected since overuse or repetitive stretching are known to trigger the release of pro-inflammatory mediators, which can induce expression of metalloproteinases and leads to collagen degradation [2]. In previous studies, magnetotherapy has shown to impact biological processes as proliferation and differentiation [3] and to modulate the inflammatory response by controlling the mast cell infiltration and healing in a rodent model upon implantation of magnetically responsive starch-PCL membranes (magSPCL) [4].

In this study we propose to establish an in vitro model by exogenous supplementation of interleukin 1β (IL-1β) to tendon resident cells (TDCs) isolated from tendon samples obtained of arthroplasty surgeries, in order to study TDCs response to inflammation with or without the actuation of a low frequency magnetic field (MF, 1h/day). Cells were collected after IL-1b stimulation (0.01 – 1ng/ml), immediately before and after MF stimulation, and at 24h, 48h and 7 days after MF and characterized for cell viability, proliferation, mRNA transcriptional levels of inflammatory cytokines and ECM remodeling proteins.

Preliminary results showed that treatment with 1ng/ml concentration of IL-1b had a positive effect on cell viability and proliferation 7 days after treatment. Although MF properties do not particularly impact cellular response, treatment with 1 ng/ml of IL-1b also influences the expression of inflammation and matrix degradation genes.

Since we also envision investigating the anti-inflammatory properties of magSPCL membranes in the modulation of TDCs response with or without IL-1B treatment (1ng/ml), ongoing studies with magnetic actuation are being performed with TDCs seeded onto these membranes.

Conference NameCHEM2NATURE Second School 2017
Date Published2017-06-05
KeywordsInflammation, magnetotherapy, Tendon
Peer reviewedno

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