Engineering patient-specific bioprinted constructs for treatment of degenerated intervertebral disc

last updated: 2019-06-04
ProjectB-FABULUS :: publications list
TitleEngineering patient-specific bioprinted constructs for treatment of degenerated intervertebral disc
Publication TypePapers in Scientific Journals
Year of Publication2019
AuthorsCosta J. B., Silva-Correia J., Ribeiro V. P., da Silva Morais A., Oliveira J. M., and Reis R. L.

Lower back pain (LBP), which is strongly associated with intervertebral disc (IVD) degeneration, is one of the
most frequently reported age- and work-related disorder in actual society, leading to a huge socio-economic impact
worldwide. The current treatments have poor clinical outcomes and do not consider each patient needs.
Thus, there is a growing interest in the potential of personalized cell-based tissue engineering (TE) approaches
aimed to regenerate the damaged IVD and efficiently restore full disc function. In this work, a bioink composed
by silk fibroin (SF) hydrogel combined with elastin was used to bioprint patient-specific substitutes mimicking
IVD ultrastructure, and particularly for the outer region of the IVD (i.e. annulus fibrosus, AF). Following a reverse
engineering approach, the proposed strategy makes use of a 3D model of AF obtained by semi-automatic morphological
segmentation from magnetic resonance imaging dataset of human IVD. SF/elastin bioprinted scaffolds
were characterized thoroughly in vitro, in terms of physico-chemical and biological performance. The bioprinted
SF/elastin scaffolds were shown to possess structural and mechanical properties similar to the native AF and
supports cell attachment and growth. Human adipose-derived stem cell cultured onto the SF/elastin bioprinted
scaffolds were shown to adhere, proliferate and maintain metabolic activity and viability up to 21days of culturing.
The implantation of custom-made SF/elastin implants that best emulate a patient AF anatomy can potentially
open up new personalized treatments for tackling IVD disorders by means of improving recovery time after
surgery and help to restore spine biofunctionality

JournalMaterials Today Communications
Date Published2019-06-04
Keywords3D printing, intervertebral disc, Patient-specific, Reverse Engineering, Silk Fibroin
Peer reviewedyes

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