The effects of platelet lysate patches on the activity of tendon-derived cells

last updated: 2018-01-17
ProjectNORTE45_3Bs - RL1 :: publications list
TitleThe effects of platelet lysate patches on the activity of tendon-derived cells
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsCosta-Almeida R., Franco A. R., Pesqueira T., Oliveira M. B., Babo P. S., Leonor I. B., Mano J. F., Reis R. L., and Gomes M. E.

Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeutic factors in patch augmentation strategies aiming at tendon repair. Developed PL patches exhibited a controlled release profile of PL proteins, including bFGF and PDGF-BB. Additionally, PL patches exhibited an antibacterial effect by preventing the adhesion, proliferation and biofilm formation by S. aureus, a common pathogen in orthopaedic surgical site infections. Furthermore, these patches supported the activity of human tendon-derived cells (hTDCs). Cells were able to proliferate over time and an up-regulation of tenogenic genes (SCXCOL1A1 and TNC) was observed, suggesting that PL patches may modify the behavior of hTDCs. Accordingly, hTDCs deposited tendon-related extracellular matrix proteins, namely collagen type I and tenascin C.

In summary, PL patches can act as a reservoir of biomolecules derived from PL and support the activity of native tendon cells, being proposed as bioinstructive patches for tendon regeneration.

JournalActa Biomaterialia
Date Published2018-01-16
KeywordsAntibacterial efficacy, controlled release, Endogenous regeneration, tendon patch, Tenogenic genes
Peer reviewedyes

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