Dual Drug Delivery System Based on pH-Sensitive Silk Fibroin/Alginate Nanoparticles Entrapped in PNIPAM Hydrogel for Treating Severe Infected Burn

last updated: 2024-02-29
ProjectFoReCaST :: publications list
TitleDual Drug Delivery System Based on pH-Sensitive Silk Fibroin/Alginate Nanoparticles Entrapped in PNIPAM Hydrogel for Treating Severe Infected Burn
Publication TypePapers in Scientific Journals
Year of Publication2020
AuthorsRezaei F., Damoogh S., Reis R. L., Kundu S. C., Mottaghitalab F., and Farokhi M.
Abstract

Herein, the pH-sensitive vancomycin (VANCO) loaded silk fibroin-sodium alginate nanoparticles (NPs) embedded in poly(N-isopropylacrylamide) (PNIPAM) hydrogel containing epidermal growth factor (EGF) are introduced for treating chronic burn wound infections. The hybrid system was developed to control the release rates of an antibiotic and growth factor for optimal treatment of burn infections. VANCO had a pH responsive release behavior from the nanoparticle (NP) and showed higher release rate in an alkaline pH compared to the neutral pH during 10 d. About 30% of EGF was also released from the hydrogel within 20 d. The released VANCO and EGF preserved their bioactivity more than ~ 80%. The suitable physico-chemical properties and cellular behaviors of PNIPAM hydrogel supported the proliferation and growth of the fibroblast cells. Furthermore, the higher re-epithelialization with good wound contraction rate, neovascular formation, and expression of transforming growth factor-beta were observed in S. aureus infected rat burn wound by using the hydrogel containing VANCO and EGF compared with untreated wounds and hydrogel alone. The wound infection was also significantly reduced in the groups treated with the hydrogels containing VANCO. Overall, in vitro and in vivo results suggested that developed hybrid system would be a promising construct to treat severe wound infection.

JournalBiofabrication
Volume13
Issue1
Pagination015005
Date Published2020-10-16
PublisherInstitute of Physics
ISSN1758-5090
DOI10.1088/1758-5090/abbb82
URLhttps://iopscience.iop.org/article/10.1088/1758-5090/abbb82
Keywordsburn injury, Drug Delivery System, Silk Fibroin
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

Back to top