Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex

last updated: 2023-11-27
ProjectCells4_IDs :: publications list
TitleDiaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex
Publication TypePapers in Scientific Journals
Year of Publication2023
AuthorsScuglia M., Frazão L. P., Miranda A., Martins A., Barbosa-Sequeira J., Coimbra D., Longatto-Filho A., Reis R. L., Nogueira-Silva C., Neves N. M., and Correia-Pinto J.


Patch repair of congenital diaphragmatic hernia (CDH) using Gore-Tex® is associated with infection, adhesions, hernia recurrence, long-term musculoskeletal sequels and poor tissue regeneration. To overcome these limitations, the performance of two novel biodegradable membranes was tested to repair CDH in a growing pig model.


Twelve male pigs were randomly assigned to 3 different groups of 4 animals each, determined by the type of patch used during thoracoscopic diaphragmatic hernia repair (Gore-Tex®, polycaprolactone electrospun membrane-PCLem, and decellularized human chorion membrane-dHCM). After 7 weeks, all animals were euthanized, followed by necropsy for diaphragmatic evaluation and histological analysis.


Thoracoscopic defect creation and diaphragmatic repair were performed without any technical difficulty in all groups. However, hernia recurrence rate was 0% in Gore-Tex®, 50% in PCLem and 100% in dHCM groups. At euthanasia, Gore-Tex® patches appeared virtually unchanged and covered with a fibrotic capsule, while PCLem and dHCM patches were replaced by either floppy connective tissue or vascularized and floppy regenerated membranous tissue, respectively.


Gore-Tex® was associated with a higher survival rate and lower recurrence. Nevertheless, the proposed biodegradable membranes were associated with better tissue integration when compared with Gore-Tex®.

JournalPediatric Surgery International
Date Published2023-11-24
PublisherSpringer Nature
KeywordsCongenital diaphragmatic hernia repair, Decellularized human chorion membrane, electrospun fibrous mesh, Gore-Tex®, Thoracoscopic surgery
Peer reviewedyes

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