Colorectal tumor-on-a-chip system: A 3D tool for precision onco-nanomedicine

last updated: 2019-05-29
ProjectFROnTHERA - RL1 :: publications list
TitleColorectal tumor-on-a-chip system: A 3D tool for precision onco-nanomedicine
Publication TypePapers in Scientific Journals
Year of Publication2019
AuthorsCarvalho M. R., Barata D., Teixeira L. M., Giselbrecht S., Reis R. L., Oliveira J. M., Truckenmüller R., and Habibovic P.

Awareness that traditional two-dimensional (2D) in vitro and nonrepresentative animal models may not completely
emulate the 3D hierarchical complexity of tissues and organs is on the rise. Therefore, posterior
translation into successful clinical application is compromised. To address this dearth, on-chip biomimetic microenvironments powered by microfluidic technologies are being developed to better capture the complexity of in vivo pathophysiology. Here, we describe a “tumor-on-a-chip” model for assessment of precision nanomedicine delivery on which we validate the efficacy of drug-loaded nanoparticles in a gradient fashion. The model validation was performed by viability studies integrated with live imaging to confirm the dose-response effect of cells exposed to the CMCht/PAMAM nanoparticle gradient. This platform also enables the analysis at the gene expression level, where a down-regulation of all the studied genes (MMP-1, Caspase-3, and Ki-67) was observed.
This tumor-on-chip model represents an important development in the use of precision nanomedicine toward
personalized treatment.

JournalScience Advances
Date Published2019-05-22
PublisherAmerican Association for the Advancement of Science.
Keywords3D, Cancer, Microfluidics, Nanoparticles
Peer reviewedyes

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