Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach

last updated: 2021-12-09
ProjectNature4Health :: publications list
TitleChitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach
Publication TypePapers in Scientific Journals
Year of Publication2021
AuthorsPiaia L., Silva S. S., Gomes J. M., Franco A. R., Fernandes E. M., Lobo F. C. M., Rodrigues L. C., Leonor I. B., Fredel M. C., Salmoria G. V., Hotza D., and Reis R. L.

Bone regeneration and natural repair are long-standing processes that can lead to uneven new
tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering
provides new approaches to manage the major burdens involved in this process. Polymeric
scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical
performance, making them suitable materials for bone regeneration solutions. The present work
aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and
evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds
have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60–170
µm, and
a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds
were able to form apatite crystals after 21 d; and they were able to support cell growth and
proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated
with silk. These outcomes further demonstrate that the developed structures are suitable
candidates to enhance bone tissue engineering.

JournalBiomedical Materials
Date Published2021-11-17
Keywordsbeta-tricalcium phosphate, BMP-2, bone regeneration, Chitosan, Silk Fibroin
RightsembargoedAccess (2 Years)
Peer reviewedyes

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