Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions

last updated: 2023-03-21
ProjectJUSThera :: publications list
TitleBiomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions
Publication TypePapers in Scientific Journals
Year of Publication2023
AuthorsBai L., Hao Z., Wang S., Zhou J., Yao S., Pei N., Zhu H., Zhang K., Reis R. L., Oliveira J. M., He J., Li D., Mao X., and Wang L.

The interactions between glioma cells and neurons are important for glioma
progression but are rarely mimicked and recapitulated in in vitro three-dimensional
(3D) models, which may affect the success rate of relevant drug research and
development. In this study, an in vitro bioprinted 3D glioma model consisting of
an outer hemispherical shell with neurons and an inner hemisphere with glioma
cells is proposed to simulate the natural glioma. This model was produced by
extrusion-based 3D bioprinting technology. The cells survival rate, morphology, and
intercellular Ca2+ concentration studies were carried out up to 5 days of culturing.
It was found that neurons could promote the proliferation of glioma cells around
them, associate the morphological changes of glioma cells to be neuron-like, and
increase the expression of intracellular Ca2+ of glioma cells. Conversely, the presence
of glioma cells could maintain the neuronal survival rate and promote the neurite
outgrowth. The results indicated that glioma cells and neurons facilitated each other
implying a symbiotic pattern established between two types of cells during the early
stage of glioma development, which were seldom found in the present artificial
glioma models. The proposed bioprinted glioma model can mimic the natural
microenvironment of glioma tissue, provide an in-depth understanding of cell–cell
interactions, and enable pathological and pharmacological studies of glioma.

JournalInternational Journal of Bioprinting
Date Published2023-03-21
KeywordsBiomimetic, Bioprinting, Glioma models
Peer reviewedyes

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