3D gellan gum hydrogel as a SERS sensing platform

last updated: 2020-01-20
ProjectBREAST-IT: Boosting Research Against Solid Tumor-Immunocompetence Tackling :: publications list
Title3D gellan gum hydrogel as a SERS sensing platform
Publication TypeComunications - Poster
Year of Publication2019
AuthorsRebelo R., Barbosa A. I., Abalde S., Wu L., Kant K., Lorenzo L., Diéguez L., Reis R. L., and Correlo V. M.

Cancer is the second high mortality disease and so, there is an urgent need for new diagnostic and treatment follow-up tools, which allow its early detection continuous monitoring. Therefore, a new generation of biosensors, with improved performance has been developed. Among the different materials to support 3D biosensors, hydrogel-based systems are considered advantageous, since they provide the ideal conditions to immobilize cells and secreted metabolites within the 3D matrix towards specific sensing.1  For instance, the real-time monitorization of lactate levels extremely important, since the accumulation of lactate in solid tumors leads to disease progression.2

Surface-enhanced Raman scattering (SERS) spectroscopy has demonstrated to be a powerful analytical tool, offering high sensitivity and selectivity, structural information and multiplexing capacity. The combination of hydrogels and nanoparticles has gained increasing attention in SERS, since the nanoparticles enable a closer interaction with the target molecules and increase the surface/volume ratio, drastically improving the biosensor performance. 3 

In this study was developed a hybrid novel material, by embedding gold-based nanoparticles into gellan gum “spongy-like” hydrogels (GG-SLH), to be used as a SERS substrate for lactate detection. The incorporation’s efficiency of the nanoparticles as well as their morphology, homogeneity and distribution were evaluated. Finally, the 3D nanoparticle loaded GG-SLH was used to detect lactate, demonstrating the great potential of this hybrid material as a sensing platform.


1         B. Sharma, M. Fernanda Cardinal, S. L. Kleinman, N. G. Greeneltch, R. R. Frontiera, M. G. Blaber, G. C. Schatz and R. P. Van Duyne, MRS Bull., 2013, 38, 615.

2         I. San-Millán and G. A. Brooks, Carcinogenesis, 2017, 38, 119–133.

3         K. Kant, S. Abalde-Cela, K. Kant and S. Abalde-Cela, Biosensors, 2018, 8, 62.

Conference NameTermStem
Date Published2019-11-08
Conference LocationBraga
KeywordsGellan Gum, plasmonic nps, sers
Peer reviewedno

Back to top