Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.

last updated: 2017-03-07
TitleUse of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Publication TypePapers in Scientific Journals
Year of Publication2011
AuthorsCarvalho P. P., Wu X., Yu G., Dietrich M., Dias I. R., Gomes M. E., Reis R. L., and Gimble J. M.
Abstract

Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to
a broad range of tissues with an ultimate goal of translating these fi ndings to clinical safety and effi cacy testing; however,
many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free
products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield,
viability and immunophenotype. ASC harvested with each trypsin product were comparable.
JournalCytotherapy
Volume13
Issue5
Pagination594-7
Date Published2011-05-02
Keywordsprotein-free products, TE
RightsopenAccess
Peer reviewedno
Statuspublished

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