A two-component pre-seeded dermal-epidermal scaffold

last updated: 2016-04-15
TitleA two-component pre-seeded dermal-epidermal scaffold
Publication TypePapers in Scientific Journals
Year of Publication2014
AuthorsMonteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., and Kohane D. S.

We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.

JournalActa Biomaterialia
Date Published2014-09-01
KeywordsAmine–aldehyde bonding, Bilayered, Hyaluronic acid, Skin defects
Peer reviewedyes

Back to top