Studying the enzymatic dyeing of textile substrates by a factorial design

The potential of enzymes as catalysts has been greatly explored over the past years and applied to obtain new and more sustainable processes. Some oxidoreductases exhibit an important feature: they have the capacity to act in certain substrates to generate or remove colour1. This enzyme is able to promote in situ polymerization of several phenolic compounds, producing coloured polymers at the substrate surface. This has encouraged its use for dyeing textile substrates as an alternative to conventional dyeing processes.

To maximize the attained results and minimize the number of assays performed, the technique of Design of Experiments (DoE) was used for planning the experiences. Design of experiments (DoE) is a tool that permits the modeling and optimization of the processes