Project | OsteoGraphy :: publications list |
Title | Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study. |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2015 |
Authors | Faia-Torres A. B., Charnley M., Goren T., Guimond-Lischer S., Rottmar M., Maniura-Weber K., Spencer N. D., Reis R. L., Textor M., and Neves N. M. |
Abstract | The use of biomaterials to direct osteogenic differentiation of human mesenchymal stem cells (hMSCs) in the absence of osteogenic supplements is thought to be part of the next generation of orthopedic implants. We previously engineered surface-roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range (0.5–4.7 μm), and mean distance between peaks (RSm) gradually varying from 214 μm to 33 μm. Here we have screened the ability of such surface-gradients of polycaprolactone to influence the expression of alkaline phosphatase (ALP), collagen type 1 (COL1) and mineralization by hMSCs cultured in dexamethasone (Dex)-deprived osteogenic induction medium (OIM) and in basal growth medium (BGM). Ra 1.53 μm/RSm 79 μm in Dex-deprived OI medium, and Ra 0.93 μm/RSm 135 μm in BGM consistently showed higher effectiveness at supporting the expression of the osteogenic markers ALP, COL1 and mineralization, compared to the tissue culture polystyrene (TCP) control in complete OIM. The superior effectiveness of specific surface-roughness revealed that this strategy may be used as a compelling alternative to soluble osteogenic inducers in orthopedic applications featuring the clinically relevant biodegradable polymer polycaprolactone. |
Journal | Acta Biomaterialia |
Volume | 28 |
Pagination | 64-75 |
Date Published | 2015-10-09 |
Publisher | Elsevier |
ISSN | 1742-7061 |
DOI | 10.1016/j.actbio.2015.09.028 |
URL | http://www.sciencedirect.com/science/article/pii/S1742706115301215 |
Keywords | Biomaterials, High-throughput screening (gradient), mesenchymal stem cells, Microstructures, osteogenesis |
Rights | restrictedAccess |
Peer reviewed | yes |
Status | published |