Novel Chitosan/ Poly (Butylene Succinate) Scaffolds For Cartilage Tissue Engineering

last updated: 2014-12-06
TitleNovel Chitosan/ Poly (Butylene Succinate) Scaffolds For Cartilage Tissue Engineering
Publication TypeConference Abstract -ISI Web of Science Indexed
Year of Publication2007
AuthorsAlves da Silva M. L., Crawford A., Mundy J., Correlo V. M., Sol P. C., Bhattacharya M., Hatton P. V., Reis R. L., and Neves N. M.

Summary of the problem: Conventional treatments for articular cartilage defects, like prosthetic surgeries have considerable limitations, specially the frequent need for revision. Tissue engineering has emerged as a potential solution for this problem, aiming at regenerating the lost or damaged tissue. This promising therapeutic route, despite the scientific efforts to repair articular cartilage loss, has not let to a complete regeneration of the functional tissue. The present work aims at evaluating the potential of 50% chitosan, 50% PBS scaffolds for a cartilage tissue engineering approach, using primary bovine articular chondrocytes.


Methods: Chondrocytes from bovine articular cartilage were isolated, according to standard procedures. Cells were seeded at a density of 6.5x106 cells/scaffold on sterile scaffolds of CPBS 50/50 using spinner flasks. After 72 hours, the hybrid constructs were changed into Petri dishes and cultured under rotational agitation for up to 4 weeks. Constructs were characterized by scanning electron microscopy, histological and immunological analysis and real time PCR.


Results and Discussion: Cells colonized the entire scaffold and were able to produce extracelular matrix. Immunolocalisation of collagens type I and II confirmed the presence of these proteins. Normalized Expression Ratio of collagen types I and II was higher in dynamic conditions when compared with control conditions (static culture). Data obtained support the conclusion that the newly developed CPBS scaffolds are very promising for cartilage tissue engineering applications.


Acknowledgements: EXPERTISSUES (NMP3-CT-2004-500238), GENOSTEM (LSHB-CT-2004-503161), Marie Curie Actions (MEST-CT-2004-008104), CARTISCAFF (POCI/SAU/BMA/58991), NATURALLY NANO (POCI/EME/58982/2004) and FCT grant attributed to M. Alves da Silva (SFRH/BD/28708/2006).

JournalTissue Engineering Part A
Date Published2007-07-01
Conference LocationLondon, UK
Keywordscartilage tissue engineering, Chitosan scaffolds
Peer reviewedyes

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