Liquidified capsules for cell immobilization coated with multilayered polyelectrolyte films

last updated: 2013-02-12
TitleLiquidified capsules for cell immobilization coated with multilayered polyelectrolyte films
Publication TypePapers in Scientific Journals
Year of Publication2011
AuthorsCosta N., Praveen S., and Mano J. F.
Abstract

Natural-derived polymers are used to coat liquid-core capsules layer by layer to encapsulate cells. Human osteoblast-like cells (SaOs-2) are encapsulated in such spherical devices using a three-step methodology: i) ionotropic gelation to produce alginate beads encapsulating the cells; ii) layer-by-layer coating using water-soluble chitosan and alginate; and iii) core liquefaction. Cells remain viable for 3 d after the encapsulation procedure, suggesting that the developed capsules possess a semipermeable, nanostructured coating. All of the capsules exhibit a spherical shape, smooth surface and liquid-core characteristics. All of the processes are conducted under mild conditions and physiological pH. We consider that the methodology employed in the development of the capsules obtained from natural-based biomaterials has potential to find applicability in the development of scaffolds or cell carriers in tissue engineering and regenerative medicine.

Journal ADVANCED ENGINEERING MATERIALS
Volume13
PaginationB218-B224
Date Published2011-06-01
PublisherJohn Wiley & Sons
DOI10.1002/adem.201080138
URLhttp://onlinelibrary.wiley.com/doi/10.1002/adem.201080138/abstract
KeywordsALGINATE, bioencapsulation, BIOMEDICAL APPLICATIONS, Chitosan, ENCAPSULATION TECHNOLOGY, L-LYSINE MICROCAPSULES, MICROENCAPSULATION, TRANSPLANTATION
RightsopenAccess
Peer reviewedyes
Statuspublished

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