Title | Liquidified capsules for cell immobilization coated with multilayered polyelectrolyte films |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2011 |
Authors | Costa N., Praveen S., and Mano J. F. |
Abstract | Natural-derived polymers are used to coat liquid-core capsules layer by layer to encapsulate cells. Human osteoblast-like cells (SaOs-2) are encapsulated in such spherical devices using a three-step methodology: i) ionotropic gelation to produce alginate beads encapsulating the cells; ii) layer-by-layer coating using water-soluble chitosan and alginate; and iii) core liquefaction. Cells remain viable for 3 d after the encapsulation procedure, suggesting that the developed capsules possess a semipermeable, nanostructured coating. All of the capsules exhibit a spherical shape, smooth surface and liquid-core characteristics. All of the processes are conducted under mild conditions and physiological pH. We consider that the methodology employed in the development of the capsules obtained from natural-based biomaterials has potential to find applicability in the development of scaffolds or cell carriers in tissue engineering and regenerative medicine. |
Journal | ADVANCED ENGINEERING MATERIALS |
Volume | 13 |
Pagination | B218-B224 |
Date Published | 2011-06-01 |
Publisher | John Wiley & Sons |
DOI | 10.1002/adem.201080138 |
URL | http://onlinelibrary.wiley.com/doi/10.1002/adem.201080138/abstract |
Keywords | ALGINATE, bioencapsulation, BIOMEDICAL APPLICATIONS, Chitosan, ENCAPSULATION TECHNOLOGY, L-LYSINE MICROCAPSULES, MICROENCAPSULATION, TRANSPLANTATION |
Rights | openAccess |
Peer reviewed | yes |
Status | published |