Layer-by-Layer Assembled Cell Instructive Nanocoatings containing Platelet Lysate

Great efforts have been made to introduce growth factors (GFs) onto 2D/3D constructs in
order to control cell behavior. Platelet Lysate (PL) presents itself as a cost-effective source of multiple
GFs and other proteins. The instruction given by a construct-PL combination will depend on how its
instructive cues are presented to the cells. The content, stability and conformation of the GFs affect
their instruction. Strategies for a controlled incorporation of PL are needed. Herein, PL was
incorporated into nanocoatings by layer-by-layer assembling with polysaccharides presenting
different sulfation degrees (SD) and charges. Heparin and several marine polysaccharides were tested
to evaluate their PL and GF incorporation capability. The consequent effects of those multilayers on
human adipose derived stem cells (hASCs) were assessed in short-term cultures. Both nature of the
polysaccharide and SD were important properties that influenced the adsorption of PL, vascular
endothelial growth factor (VEGF), fibroblast growth factor b (FGFb) and platelet derived growth factor
(PDGF). The sulfated polysaccharides-PL multilayers showed to be efficient in the promotion of
morphological changes, serum-free adhesion and proliferation of high passage hASCs (P>5). These
biomimetic multilayers promise to be versatile platforms to fabricate instructive devices allowing a
tunable incorporation of PL.