Title | Effect of cell density on mesenchymal stem cells aggregation in RGD-alginate 3D matrices under osteoinductive conditions |
Publication Type | Papers in Scientific Journals |
Year of Publication | 2014 |
Authors | Maia F. R., Lourenço A. F., Granja P. L., Gonçalves R. M., and Barrias C. C. |
Abstract | Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered “normal” activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell–cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering. |
Journal | Macromolecular Bioscience |
Volume | 14 |
Issue | 6 |
Pagination | 659-771 |
Date Published | 2014-02-28 |
DOI | 10.1002/mabi.201300567 |
URL | http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300567/abstract;jsessionid=902F43BD5677D920D14697F6503D3D0D.f03t02 |
Keywords | alginate hydrogels, encapsulation, self-assembly, Tissue engineering |
Rights | openAccess |
Peer reviewed | yes |
Status | published |