Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications

Cells microencapsulated in biocompatible semi-permeable polymeric
membranes are effective as cell delivery systems while protecting the host
against immune responses. In this study, cell encapsulation membranes were
prepared based on carrageenan and alginate, two natural cationic polymers.
Different formulations/conditions were explored to optimize the microcapsules
which were characterized with respect to their morphology, mechanical stability,
and cytotoxicity. Spherical-shaped microcapsules were obtained from all the
polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited
the best stability and permeability, and therefore, these were selected for
the cell encapsulation. These capsules provided an environment that supported
cell proliferation and have the potential for tissue engineering as well as other
cell-based therapy applications.