Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation

last updated: 2016-09-05
TitleContinuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation
Publication TypePapers in Scientific Journals
Year of Publication2016
AuthorsRibeiro V. P., Castro F., Ferreira A., Oliveira A. L., Reis R. L., Teixeira J. A., and Rocha F.
Abstract

This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physico- chemical characterization of the synthesized HAp suggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μg ml1), although its cellular uptake behavior. Therefore, the prepared HAp shows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties. 

JournalMaterials Research Express
Volume3
Issue7
Date Published2016-07-21
PublisherIOP Publishing Ltd
ISSN2053-1591
DOI10.1088/2053-1591/3/7/075404
URLhttp://iopscience.iop.org/article/10.1088/2053-1591/3/7/075404
KeywordsCalcium phosphates, mineralization, nanohydroxyapatite, oscillatory flow reactor, SBF
RightsopenAccess
Peer reviewedyes
Statuspublished

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