Bioinspired Microfabricated Scaffolds for Tendon Tissue Engineering

last updated: 2013-10-15
TitleBioinspired Microfabricated Scaffolds for Tendon Tissue Engineering
Publication TypeComunication - Oral
Year of Publication2013
AuthorsRodrigues M. T., Gonçalves A. I., Reis R. L., and Gomes M. E.

Tendons are composed of packed collagen fiber bundles aligned parallel to the longitudinal tendon axis and surrounded by a sheath1 that improves friction-free gliding. This study aims to bioengineer fiber bundled
scaffolds to promote an aligned orientation of tendon cells as in native functional tissue. Moreover, these scaffolds were embedded in agarose to resemble tendon sheath or in a platelet lysate (PL)-rich agarose coating. PLs were chosen as a natural source of growth factors known to promote tissue regeneration2. Fiber
scaffolds made of a blend of starch and polycaprolactone (SPCL) were designed by 3D printing and rolled into bundle-like structures. Tendon cells obtained from surgery surplus were expanded in basic medium. 24h after seeding (2x105cells/scaffold) constructs were dip-coated in an agarose solution (2% in PBS) or in a PL (5mg/mL)-agarose solution for 7 days.

Conference NameTERMIS-EU
Date Published2013-06-17
Conference LocationIstanbul, Turkey
KeywordsAgarose coating, Platelet lysate, SPCL scaffolds, Tendon TE
Peer reviewedyes

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