Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs

last updated: 2015-08-06
TitleAdditively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
Publication TypePapers in Scientific Journals
Year of Publication2015
AuthorsCosta P. F., Hutmacher D. W., Theodoropoulos C., Gomes M. E., Reis R. L., and Vaquette C.

The ability to test large arrays of cell and biomaterial combinations in 3D environments is still rather limited in the context of tissue engineering and regenerative medicine. This limitation can be generally addressed by employing highly automated and reproducible methodologies. This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface-coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP-coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.

JournalAdvanced Healthcare Materials
Date Published2015-04-22
PublisherWILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywordsadditive manufacturing, bioreactors, regenerative medicine, Tissue engineering
Peer reviewedyes

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