@article {20718, title = {Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications}, journal = {Acta Biomaterialia}, volume = {141}, year = {2022}, month = {2022-01-10 00:00:00}, pages = {123-131}, publisher = {Elsevier}, abstract = {

The host immunologic response to a specific material is a critical aspect when considering it for clinical implementation. Collagen and gelatin extracted from marine sources have been proposed as\ biomaterials\ for tissue engineering applications, but there is a lack of information in the literature about their\ immunogenicity. In this work, we evaluated the immune response to collagen and/or gelatin from blue shark and codfish, previously extracted and characterized. After endotoxin evaluation, bone marrow-derived macrophages were exposed to the materials and a panel of pro- and anti-inflammatory cytokines were evaluated both for protein quantification and gene expression. Then, the impact of those materials in the host was evaluated through peritoneal injection in C57BL/6 mice. The results suggested shark collagen as the less immunogenic material, inducing low expression of pro-inflammatory cytokines as well as inducible\ nitric oxide\ synthase (encoded by\ Nos2) and high expression of\ Arginase\ 1 (encoded by\ Arg1). Although shark gelatin appeared to be the material with higher pro-inflammatory expression, it also presents a high expression of IL-10 (anti-inflammatory cytokine) and Arginase (both markers for M2-like macrophages). When injected in the peritoneal cavity of mice, our materials demonstrated a transient recruitment of neutrophil, being almost non-existent after 24 hours of injection. Based on these findings, the studied collagenous materials can be considered interesting biomaterial candidates for regenerative medicine as they may induce an activation of the M2-like macrophage population, which is involved in suppressing the inflammatory processes promoting tissue remodeling.

}, keywords = {Anti-inflammatory macrophages, immunogenicity, M2-like macrophages, marine biomaterials, type I collagen}, issn = {1742-7061}, doi = {10.1016/j.actbio.2022.01.009}, url = {https://doi.org/10.1016/j.actbio.2022.01.009}, author = {Alves, A. L. and Costa-Gouveia, J. and Vieira de Castro, J. and Sotelo, C. G. and V{\'a}zquez, J. A. and P{\'e}rez Mart{\'\i}n, R. I. and Torrado, E. and Neves, N. M. and Reis, R. L. and Castro, A. G. and Silva, T. H.} }