@article {20562, title = {Fucoidan/chitosan nanoparticles functionalized with anti-ErbB-2 target breast cancer cells and impair tumor growth in vivo}, journal = { International Journal of Pharmaceutics}, volume = {600}, year = {2021}, month = {2021-05-01 00:00:00}, pages = {120548}, abstract = {

The work herein presented reports the development of fucoidan/chitosan nanoparticles (NPs) loaded with gemcitabine and functionalized with ErbB-2 antibody at their surface (NPs + Gem + Ab). The maximum immobilization of ErbB-2 on NPs{\textquoteright} surface was set at 10 μg mL-1\ and resulted in NPs with a size around 160 nm, a polydispersity index of 0.18, and a zeta potential of 21 mV. ErbB-2 is overexpressed in some subtypes of breast cancers, and the targeting capability of the NPs + Gem + Ab system was confirmed by an increased cellular uptake of SKBR3 cells (ErbB-2 positive) when compared to MDA-MB-231 (ErbB-2 negative). To validate the targeting efficacy of NPs + Gem + Ab, a co-culture system with human endothelial and SKBR3 cells was established. Cytotoxic effects over endothelial cells were similar for all the tested conditions (between 25 and 30\%). However, the NPs + Gem + Ab system presented increased toxicity over breast cancer cells, above 80\% after 24 h, when compared to free Gem and NPs + Gem (around 15\% and 20\%, respectively). In vivo studies demonstrated that the developed targeting system significantly reduced tumor growth and the appearance of lung metastasis compared to untreated controls. In summary, the efficacy of the NPs + Gem + Ab system to target cancer cells was established and validated both in vitro and in vivo, being a compelling alternative strategy to current chemotherapeutic approaches.

}, keywords = {breast cancer, Marine polymeric nanoparticles, Targeting}, issn = {0378-5173}, doi = {10.1016/j.ijpharm.2021.120548}, url = {https://www.sciencedirect.com/science/article/pii/S0378517321003537?via\%3Dihub}, author = {Oliveira, C. and Gon{\c c}alves, C. S. and Martins, E. P. and Neves, N. M. and Reis, R. L. and Costa, B. M. and Silva, T. H. and Martins, A.} }