@article {20536, title = {Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges}, journal = {Frontiers in Marine Science}, volume = {8}, year = {2021}, month = {2021-05-17 00:00:00}, pages = {637810}, publisher = {Frontiers Media S.A.}, abstract = {

Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalysed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI) and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI- and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ~1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity towards L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE).

}, keywords = {bioactivity, Bioceramics, biosilica, deep-sea sponges, tissue engineering.}, issn = {2296-7745}, doi = {10.3389/fmars.2021.637810}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/full}, author = {Dudik, O. and Amorim, S. and Xavier, J. R. and Rapp, H. T. and Silva, T. H. and Pires, R. A. and Reis, R. L.}, editor = {Kenchington, E.} }