@article {20479,
	title = {A microfluidic platform for the investigation of nanoparticles cellular uptake and cancer cells tracking in real-time},
	booktitle = {TERMSTEM 2016},
	year = {2016},
	month = {2016-10-31 00:00:00},
	address = {Vila Flor - Guimar{\~a}es, Portugal},
	abstract = {<p>In this study, we aim to endorse an improved platform composed of labeled dendrimer nanoparticles and a microfluidic\&nbsp;device for real-time monitoring of cells fate. Carboxymethyl-chitosan/poly(amidoamine) (CMCht/PAMAM)\&nbsp;dendrimer nanoparticles were synthetized, labeled with the fluorescent label probe Fluorescein-5(6)-isothiocyanate\&nbsp;(FITC) and characterized using Transmission electron microscopy, Atomic force microscopy, Dynamic light scattering,\&nbsp;and Differential scanning calorimetry. Different cell types were cultured{\textellipsis}..After culturing HeLa (Cervical<br>Carcinoma), HCT-116 (Colon Carcinoma) and U87MG (Glioblastoma) in the presence of these nanoparticles, cell\&nbsp;viability and internalization efficiency in static (standard cultures) and dynamic (microfluidic cultures) conditions were\&nbsp;investigated. Results showed that cancer cells cultured with nanoparticles in dynamic conditions were viable and\&nbsp;presented higher internalization levels as compared to static 2D cultures. This work demonstrated that the proposed\&nbsp;microfluidic platform allowed real-time cell monitoring, which, upon more studies, namely the assessment of the drug\&nbsp;release effect could be used in the future for the screening of new therapeutics and applied in the treatment of\&nbsp;amyotrophic lateral sclerosis.</p>},
	keywords = {Cancer, Dendrimers, Microfluidics},
	author = {Carvalho, M. R. and Maia, F. R. and Silva-Correia, J. and Costa, B. M. and Reis, R. L. and Oliveira, J. M.}
}
