@article {20438,
	title = {Liposomes embedded in layer by layer constructs as simplistic extracellular vesicles transfer model},
	journal = {Materials Science and Engineering C},
	volume = {121},
	year = {2021},
	month = {2021-01-13 00:00:00},
	pages = {111813},
	publisher = {Elsevier},
	abstract = {<p>Extracellular vesicles (EVs) are particles originating from the exfoliation of the cellular membrane. They are involved in cell-to-cell and cell-to-matrix signaling, exchange of bioactive molecules, tumorigenesis and metastasis, among others. To mitigate the limited understanding of EVs transfer phenomena, we developed a simplistic model that mimics EVs and their interactions with cells and the extracellular matrix. The proposed model is a layer-by-layer (LbL) film built from the polycationic poly-L-lysine (PLL) and the glycosaminoglycan hyaluronic acid (HA) to provide ECM mimicry. Positively charged 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and N1,N1,N14,N14-tetramethyl-N1,N14-ditetradecyltetradecane-1,14-diaminium dibromide (GS14) liposomes were embedded in this construct to act as EVs analogs. To simulate EVs carrying substances, Nile Red was loaded as a model of lipophilic cargo molecules. The integration of each component was followed by quartz-crystal microbalance measurements, which confirmed the immobilization of intact liposomes on the underlying (PLL/HA)3 soft film. The release of Nile Red from liposomes either embedded in the LbL construct or exposed at its surface revealed a fast first order release. This system was validated as a model for EV/cell interactions by incubation with breast cancer cells MDA-MB-231. We observed higher internalization for embedded liposomes when compared with surface-exposed ones, showcasing that the ECM mimic layers do not constitute a barrier to liposome/cell interactions but favor them.</p>},
	keywords = {cell communication, Extracellular matrix, Glycosaminoglycans, layer-by-layer, Liposomes, Supported vesicle layers},
	issn = {0928-4931},
	doi = {10.1016/j.msec.2020.111813},
	url = {https://www.sciencedirect.com/science/article/pii/S0928493120337322},
	author = {Dom{\'\i}nguez-Arca, V. and Costa, R. R. and Carvalho, A. M. and Taboada, P. and Reis, R. L. and Prieto, G. and Pashkuleva, I.}
}
