@article {20326,
	title = {Surface biofunctionalization to improve the efficacy of biomaterial substrates to be used in regenerative medicine},
	journal = {Materials Horizons},
	year = {2020},
	month = {2020-05-26 00:00:00},
	pages = {1-18},
	publisher = {The Royal Society of Chemistry},
	abstract = {<p><span>Surface biofunctionalization has emerged in the past decade as a rising tool in Tissue Engineering and Regenerative Medicine (TERM) approaches. The development of biomaterial substrates with regenerative properties is challenging, mainly due to the requirement of obtaining a surface that promotes cell attachment, proliferation and differentiation. Biomolecules, namely extracellular matrix proteins and growth factors, play a key role in the wound healing cascade, mediating a wide range of cellular activities. Therefore, the immobilization of such biomolecules at the surface of biomaterials has a remarkable interest for TERM strategies. Numerous variants can be used to biofunctionalize the surface of biomaterials, being classified as non-covalent and covalent immobilization strategies. Importantly, the maintenance of stable/available bioactive factors needs also to be considered. In this review, a wide range of bioactive molecules and different biofunctionalization strategies will be discussed, as well as the most relevant results achieved in the novel and most promising strategies.</span></p>},
	keywords = {bioactive molecules, Biomaterials Substrate, growth factors, surface functionalization, Surface Immobilization, tissue engineering and regenerative medicine},
	issn = {2051-6347},
	doi = {10.1039/D0MH00542H},
	url = {http://dx.doi.org/10.1039/D0MH00542H},
	author = {Casanova, M. R. and Reis, R. L. and Martins, A. and Neves, N. M.}
}
