@article {20039, title = {HYDROGELS BASED ON GELLAN GUM AND ELASTIN FOR WOUND HEALING }, journal = {1st Discoveries Forum on Regenerative and Precision Medicine}, year = {2019}, month = {2019-09-25 00:00:00}, address = {Porto, Portugal}, abstract = {

The development of novel hydrogels combining different concentrations of gellan gum and elastin were investigated. Hydrogels were submitted to morphological analysis using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS). The elastic performance was investigated through the mechanical properties of hydrogels using compression tests after incubation with PBS azide at 37 {\textordmasculine}C for 0, 7 and 14 days. Results obtained from SEM and TEM confirmed the presence of elastin in the hydrogels, as well as the protein{\textquoteright}s characteristic fibrils, responsible for the increase in the elasticity of biological tissues. The EDS revealed clear peaks of sulphur in the hydrogels with elastin fibrils, further confirming those results. The mechanical properties showed the nonlinear mechanical behaviour of the hydrogels and an increase of the compressive modulus and compressive strength as the amount of polymer in the hydrogel increased. Incorporation of elastin resulted in increased compressive modulus and elongation capacity of the hydrogels. The measurements performed demonstrated that the hydrogels are stable along the time. Our results indicate that the mechanical properties of hydrogels can be tuned to develop new biomaterials to address the required mechanical properties of elastic tissues.

Acknowledgements:

This research was supported by the Portuguese Foundation of Science and Technology under the project Dressing4Scars (ERA-NET/CSA/JPI/M-ERANET). The authors would like to acknowledge The Discoveries Centre for Regenerative and Precision Medicine and the funds for POCI-01-0145-FEDER-007038-UMINHO/BPD/44/2016 (LPS), FCT/MCTES PD/BD/135248/2017 (DPR) and M-ERA-NET2/0013/2016 (MGF) grants. The authors thank Dr Santiago Sevillano (Leica Nanotechnology) the hydrogels preparation for SEM.

}, keywords = {Elastin, Gellan Gum, hydrogel}, author = {Fernandes, M. G. and Reis, D. P. and da Silva, L. P. and Reis, R. L. and Marques, A. P.} }