@article {19888, title = {Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio}, journal = {Carbohydrate Polymers}, volume = {222}, year = {2019}, month = {2019-06-18 00:00:00}, pages = {1149992}, publisher = {Elsevier}, abstract = {

MO245 exopolysaccharide (EPS) was produced in laboratory conditions from\ Vibrio\ genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit {\textrightarrow}4)-β-D-GlcpA-(1{\textrightarrow}4)-α-D-GalpNAc-(1{\textrightarrow}3)-β-D-GlcpNAc-(1{\textrightarrow}4)-β-D-GlcpA-(1{\textrightarrow} containing covalently-linked 5\% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 {\textpm} 4 kDa (PDI, 1.42 {\textpm} 0.01), 6.7 {\textpm} 0.3 dl/g and 56 {\textpm} 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1{\textrightarrow}3 and 1{\textrightarrow}4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.

}, keywords = {exopolysaccharide, Hyaluronic acid}, issn = {0144-8617}, doi = {10.1016/j.carbpol.2019.114999}, url = {https://doi.org/10.1016/j.carbpol.2019.114999}, author = {Martin-Pastor, M. and Ferreira, A. S. and Moppert, X. and Nunes, C. and Coimbra, M. A. and Reis, R. L. and Guezennec, J. and Novoa-Carvallal, R.}, editor = {Elsevier} }